Type: HPLC Application #: 100470
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV 280nm, absorbance ranges: 0 - 7min 0.08 AUFS, 7 - 50min 0.04 AUFS Conditions: eluent: tetrahydrofuran - methanol - water - acetic acid (25:30:45:0.1, v/v), pH 5.5 with ammonium hydroxide, aqueous phase with 0.1% EDTA to prevent binding to the column of cations, which could lead to excessive retention of sulphidopeptide leukotrienes, flow rate: 0.9ml/min Authors: Verhagen J Source: Prostaglandins Leukotrienes and Medicine 13 (1984)
Details
Legend: Reversed phase chromatogram of synthetic LTB4, LTC4, LTD4 and the leukotrienes
produced by human PMNLs,
THETE,
LTC4,
11t-LTC4,
PGB2 (int. std.),
LTD4,
6t-LTB4 + 12epi,6t-LTB4,
LTB4,
12epi,6